1AZO

DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI

1AZO の概要

• エントリーDOI: 10.2210/pdb1azo/pdb
• 分子名称: MUTH, 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: endonuclease, muth, dna repair, hydrolase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26531.12

構造登録者

Yang, W. (登録日: 1997-11-19, 公開日: 1998-05-20, 最終更新日: 2024-10-16)

主引用文献

Ban, C.,Yang, W.
Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases.
EMBO J., 17:1526-1534, 1998

PubMed Abstract: MutS, MutL and MutH are the three essential proteins for initiation of methyl-directed DNA mismatch repair to correct mistakes made during DNA replication in Escherichia coli. MutH cleaves a newly synthesized and unmethylated daughter strand 5' to the sequence d(GATC) in a hemi-methylated duplex. Activation of MutH requires the recognition of a DNA mismatch by MutS and MutL. We have crystallized MutH in two space groups and solved the structures at 1.7 and 2.3 A resolution, respectively. The active site of MutH is located at an interface between two subdomains that pivot relative to one another, as revealed by comparison of the crystal structures, and this presumably regulates the nuclease activity. The relative motion of the two subdomains in MutH correlates with the position of a protruding C-terminal helix. This helix appears to act as a molecular lever through which MutS and MutL may communicate the detection of a DNA mismatch and activate MutH. With sequence homology to Sau3AI and structural similarity to PvuII endonuclease, MutH is clearly related to these enzymes by divergent evolution, and this suggests that type II restriction endonucleases evolved from a common ancestor.

PubMed: 9482749
DOI: 10.1093/emboj/17.5.1526

実験手法

X-RAY DIFFRACTION (1.7 Å)