1AZF

CHICKEN EGG WHITE LYSOZYME CRYSTAL GROWN IN BROMIDE SOLUTION

1AZF の概要

• エントリーDOI: 10.2210/pdb1azf/pdb
• 分子名称: LYSOZYME, BROMIDE ION (3 entities in total)
• 機能のキーワード: hydrolase, o-glycosyl hydrolase
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14650.78

構造登録者

Lim, K.,Nadarajah, A.,Forsythe, E.L.,Pusey, M.L. (登録日: 1997-11-17, 公開日: 1998-02-25, 最終更新日: 2024-10-23)

主引用文献

Lim, K.,Nadarajah, A.,Forsythe, E.L.,Pusey, M.L.
Locations of bromide ions in tetragonal lysozyme crystals.
Acta Crystallogr.,Sect.D, 54:899-904, 1998

PubMed Abstract: Anions have been shown to play a dominant role in the crystallization of chicken egg-white lysozyme from salt solutions. Previous studies employing X-ray crystallography have found one chloride ion binding site in the tetragonal crystal form of the protein and four nitrate ion binding sites in the monoclinic form. In this study the anion positions in the tetragonal form were determined from the difference Fourier map obtained from lysozyme crystals grown in bromide and chloride solutions. Five possible anion-binding sites were found in this manner. Some of these sites were in pockets containing basic residues while others were near neutral, but polar, residues. The sole chloride ion binding site found in previous studies was confirmed, while four further sites were found which corresponded to the four binding sites found for nitrate ions in monoclinic crystals. The study suggests that most of the anion-binding sites in lysozyme remain unchanged even when different anions and different crystal forms of lysozyme are employed.

PubMed: 9757106
DOI: 10.1107/S0907444998002844

実験手法

X-RAY DIFFRACTION (1.8 Å)