1AXM

HEPARIN-LINKED BIOLOGICALLY-ACTIVE DIMER OF FIBROBLAST GROWTH FACTOR

1AXM の概要

• エントリーDOI: 10.2210/pdb1axm/pdb
• 分子名称: ACIDIC FIBROBLAST GROWTH FACTOR, 2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose, 2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid, ... (4 entities in total)
• 機能のキーワード: human acidic fibroblast growth factor, heparin decasaccharide, growth factor
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 96434.20

構造登録者

DiGabriele, A.D.,Lax, I.,Chen, D.I.,Svahn, C.M.,Jaye, M.,Schlessinger, J.,Hendrickson, W.A. (登録日: 1997-10-16, 公開日: 1998-04-22, 最終更新日: 2024-10-23)

主引用文献

DiGabriele, A.D.,Lax, I.,Chen, D.I.,Svahn, C.M.,Jaye, M.,Schlessinger, J.,Hendrickson, W.A.
Structure of a heparin-linked biologically active dimer of fibroblast growth factor.
Nature, 393:812-817, 1998

PubMed Abstract: The fibroblast growth factors (FGFs) form a large family of structurally related, multifunctional proteins that regulate various biological responses. They mediate cellular functions by binding to transmembrane FGF receptors, which are protein tyrosine kinases. FGF receptors are activated by oligomerization, and both this activation and FGF-stimulated biological responses require heparin-like molecules as well as FGF. Heparins are linear anionic polysaccharide chains; they are typically heterogeneously sulphated on alternating L-iduronic and D-glucosamino sugars, and are nearly ubiquitous in animal tissues as heparan sulphate proteoglycans on cell surfaces and in the extracellular matrix. Although several crystal structures have been described for FGF molecules in complexes with heparin-like sugars, the nature of a biologically active complex has been unknown until now. Here we describe the X-ray crystal structure, at 2.9 A resolution, of a biologically active dimer of human acidic FGF in a complex with a fully sulphated, homogeneous heparin decassacharide. The dimerization of heparin-linked acidic FGF observed here is an elegant mechanism for the modulation of signalling through combinatorial homodimerization and heterodimerization of the 12 known members of the FGF family.

PubMed: 9655399
DOI: 10.1038/31741

実験手法

X-RAY DIFFRACTION (3 Å)