1AXD

STRUCTURE OF GLUTATHIONE S-TRANSFERASE-I BOUND WITH THE LIGAND LACTOYLGLUTATHIONE

1AXD の概要

• エントリーDOI: 10.2210/pdb1axd/pdb
• 分子名称: GLUTATHIONE S-TRANSFERASE I, LACTOYLGLUTATHIONE (3 entities in total)
• 機能のキーワード: transferase, herbicide detoxification, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• 由来する生物種: Zea mays
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47420.56

構造登録者

Neuefeind, T.,Huber, R.,Dasenbrock, H.,Prade, L.,Bieseler, B. (登録日: 1997-10-15, 公開日: 1998-10-28, 最終更新日: 2023-11-15)

主引用文献

Neuefeind, T.,Huber, R.,Dasenbrock, H.,Prade, L.,Bieseler, B.
Crystal structure of herbicide-detoxifying maize glutathione S-transferase-I in complex with lactoylglutathione: evidence for an induced-fit mechanism.
J.Mol.Biol., 274:446-453, 1997

PubMed Abstract: Glutathione S-transferases (GSTs) -I and -III are involved in herbicide metabolism in maize and have been intensively studied. Starting with plant tissue from Zea mays var. mutin recombinant GST-I was prepared by heterologous expression in Escherichia coli. The enzyme was crystallized in the presence of lactoylglutathione, a ligand formerly never observed in a GST structure and known as an intermediate of the pharmacologically relevant glyoxalase system. The crystal structure of GST-I has been determined at 2.5 A resolution and exhibits the GST-typical dimer of two identical subunits, each consisting of 214 residues. Compared with other plant GSTs the three-dimensional structure of GST-I primarily shows structural differences in the hydrophobic substrate binding site, the linker segment and the C-terminal region. Furthermore, a comparison of the ligand-bound GST-I structure with the apo structure of GST-III indicates the movement of a ten-residue loop upon binding of the ligand to the active site. This is the first structure-based evidence for an induced fit mechanism of glutathione S-transferases, which has previously been postulated for class pi enzymes. Together with GST-III, GST-I may explain herbicide resistance and selectivity in maize as well as in other agronomic relevant crops.

PubMed: 9417926
DOI: 10.1006/jmbi.1997.1402

実験手法

X-RAY DIFFRACTION (2.5 Å)