1AXC

HUMAN PCNA

1AXC の概要

• エントリーDOI: 10.2210/pdb1axc/pdb
• 分子名称: PCNA, P21/WAF1 (3 entities in total)
• 機能のキーワード: dna, replication, processivity, oncogene, waf1, cip1, complex (dna-binding protein-dna), complex (dna-binding protein-dna) complex, complex (dna-binding protein/dna)
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P12004; Cytoplasm: P38936
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 94724.95

構造登録者

Gulbis, J.M.,Kuriyan, J. (登録日: 1997-10-14, 公開日: 1998-01-28, 最終更新日: 2024-04-03)

主引用文献

Gulbis, J.M.,Kelman, Z.,Hurwitz, J.,O'Donnell, M.,Kuriyan, J.
Structure of the C-terminal region of p21(WAF1/CIP1) complexed with human PCNA.
Cell(Cambridge,Mass.), 87:297-306, 1996

PubMed Abstract: The crystal structure of the human DNA polymerase delta processivity factor PCNA (proliferating cell nuclear antigen) complexed with a 22 residue peptide derived from the C-terminus of the cell-cycle checkpoint protein p21(WAF1/CIP1) has been determined at 2.6 angstrom resolution. p21 binds to PCNA in a 1:1 stoichiometry with an extensive array of interactions that include the formation of a beta sheet with the interdomain connector loop of PCNA. An intact trimeric ring is maintained in the structure of the p21-PCNA complex, with a central hole available for DNA interaction. The ability of p21 to inhibit the action of PCNA is therefore likely to be due to its masking of elements on PCNA that are required for the binding of other components of the polymerase assembly.

PubMed: 8861913
DOI: 10.1016/S0092-8674(00)81347-1

実験手法

X-RAY DIFFRACTION (2.6 Å)