1AVB

ARCELIN-1 FROM PHASEOLUS VULGARIS L

1AVB の概要

• エントリーDOI: 10.2210/pdb1avb/pdb
• 分子名称: ARCELIN-1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: lectin-like glycoprotein, plant defense, insecticidal activity, lectin
• 由来する生物種: Phaseolus vulgaris
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51894.87

構造登録者

Mourey, L.,Pedelacq, J.D.,Fabre, C.,Rouge, P.,Samama, J.P. (登録日: 1997-09-15, 公開日: 1998-10-14, 最終更新日: 2024-11-13)

主引用文献

Mourey, L.,Pedelacq, J.D.,Birck, C.,Fabre, C.,Rouge, P.,Samama, J.P.
Crystal structure of the arcelin-1 dimer from Phaseolus vulgaris at 1.9-A resolution.
J.Biol.Chem., 273:12914-12922, 1998

PubMed Abstract: Arcelin-1 is a glycoprotein from kidney beans (Phaseolus vulgaris) which displays insecticidal properties and protects the seeds from predation by larvae of various bruchids. This lectin-like protein is devoid of monosaccharide binding properties and belongs to the phytohemagglutinin protein family. The x-ray structure determination at 1.9-A resolution of native arcelin-1 dimers, which correspond to the functional state of the protein in solution, was solved using multiple isomorphous replacement and refined to a crystallographic R factor of 0.208. The three glycosylation sites on each monomer are all covalently modified. One of these oligosaccharide chains provides interactions with protein atoms at the dimer interface, and another one may act by preventing the formation of higher oligomeric species in the arcelin variants. The dimeric structure and the severe alteration of the monosaccharide binding site in arcelin-1 correlate with the hemagglutinating properties of the protein, which are unaffected by simple sugars and sugar derivatives. Sequence analysis and structure comparisons of arcelin-1 with the other insecticidal proteins from kidney beans, arcelin-5, and alpha-amylase inhibitor and with legume lectins, yield insights into the molecular basis of the different biological functions of these proteins.

PubMed: 9582323
DOI: 10.1074/jbc.273.21.12914

実験手法

X-RAY DIFFRACTION (1.9 Å)