1AQZ

CRYSTAL STRUCTURE OF A HIGHLY SPECIFIC ASPERGILLUS RIBOTOXIN, RESTRICTOCIN

Summary for 1AQZ

Entry DOI	10.2210/pdb1aqz/pdb
Descriptor	RESTRICTOCIN, PHOSPHATE ION (3 entities in total)
Functional Keywords	ribotoxin, ribosome-inactivating protein, protein-rna specific interaction, laue diffraction, cell-entry activity
Biological source	Aspergillus restrictus
Cellular location	Secreted: P67876
Total number of polymer chains	2
Total formula weight	34062.70
Authors	Yang, X.,Moffat, K. (deposition date: 1997-08-04, release date: 1997-11-12, Last modification date: 2024-10-30)
Primary citation	Yang, X.,Moffat, K. Insights into specificity of cleavage and mechanism of cell entry from the crystal structure of the highly specific Aspergillus ribotoxin, restrictocin. Structure, 4:837-852, 1996 Cited by PubMed Abstract: Restriction, a highly specific ribotoxin made by the fungus Aspergillus restrictus, cleaves a single phosphodiester bond in the 28S RNA of eukaryotic ribosomes, inhibiting protein synthesis. The sequence around this cleavage site is a binding site for elongation factors, and is conserved in all cytoplasmic ribosomes. The catalytic mechanism of restrictocin and the reasons for its high substrate specificity are unknown. No structure has been determined for any other member of the Aspergillus ribotoxin family. PubMed: 8805570 DOI: 10.1016/S0969-2126(96)00090-1 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.7 Å)

Structure validation