1AQB

RETINOL-BINDING PROTEIN (RBP) FROM PIG PLASMA

1AQB の概要

• エントリーDOI: 10.2210/pdb1aqb/pdb
• 分子名称: RETINOL-BINDING PROTEIN, CADMIUM ION, RETINOL, ... (4 entities in total)
• 機能のキーワード: retinol transport, retinoids, vitamin a, cadmium ion
• 由来する生物種: Sus scrofa domestica (domestic pig)
• 細胞内の位置: Secreted : P27485
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21582.52

構造登録者

Zanotti, G.,Panzalorto, M.,Marcato, A.,Malpeli, G.,Folli, C.,Berni, R. (登録日: 1997-07-28, 公開日: 1998-01-28, 最終更新日: 2024-11-20)

主引用文献

Zanotti, G.,Panzalorto, M.,Marcato, A.,Malpeli, G.,Folli, C.,Berni, R.
Structure of pig plasma retinol-binding protein at 1.65 A resolution.
Acta Crystallogr.,Sect.D, 54:1049-1052, 1998

PubMed Abstract: The crystal structure of pig plasma retinol-binding protein (RBP) has been determined at 1.65 A resolution. The space group is P212121, with a = 45.81 (4), b = 53.14 (5), c = 72.97 (8) A and one protein molecule in the asymmetric unit. The structure has been solved using the molecular replacement method and refined with restrained least squares to an R factor of 0.1844 and an Rfree of 0.237 for 18 874 and 1001 independent reflections, respectively. The relatively high resolution structure of pig holoRBP has revealed some new structural details. Moreover, it has provided a description of the binding site for Cd2+, a metal ion which is required for protein crystallization. The hepta-coordination of the RBP-bound cadmium ion involves different residues of two symmetry-related RBP molecules, consistent with the participation of the cation in intermolecular interactions that in turn promote protein crystallization.

PubMed: 9757135
DOI: 10.1107/S0907444998002303

実験手法

X-RAY DIFFRACTION (1.65 Å)