1APL

CRYSTAL STRUCTURE OF A MAT-ALPHA2 HOMEODOMAIN-OPERATOR COMPLEX SUGGESTS A GENERAL MODEL FOR HOMEODOMAIN-DNA INTERACTIONS

1APL の概要

• エントリーDOI: 10.2210/pdb1apl/pdb
• 分子名称: DNA (5'-D(*AP*CP*AP*TP*GP*TP*AP*AP*TP*TP*CP*AP*TP*TP*TP*AP*C P*AP*CP*GP*C)-3'), DNA (5'-D(*TP*GP*CP*GP*TP*GP*TP*AP*AP*AP*TP*GP*AP*AP*TP*TP*A P*CP*AP*TP*G)-3'), PROTEIN (MAT-ALPHA2 HOMEODOMAIN) (3 entities in total)
• 機能のキーワード: protein-dna complex, double helix, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Nucleus: Q6B2C0
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 32429.00

構造登録者

Wolberger, C.,Vershon, A.K.,Liu, B.,Johnson, A.D.,Pabo, C.O. (登録日: 1993-10-04, 公開日: 1993-10-21, 最終更新日: 2024-02-07)

主引用文献

Wolberger, C.,Vershon, A.K.,Liu, B.,Johnson, A.D.,Pabo, C.O.
Crystal structure of a MAT alpha 2 homeodomain-operator complex suggests a general model for homeodomain-DNA interactions.
Cell(Cambridge,Mass.), 67:517-528, 1991

PubMed Abstract: The MAT alpha 2 homeodomain regulates the expression of cell type-specific genes in yeast. We have determined the 2.7 A resolution crystal structure of the alpha 2 homeodomain bound to a biologically relevant DNA sequence. The DNA in this complex is contacted primarily by the third of three alpha-helices, with additional contacts coming from an N-terminal arm. Comparison of the yeast alpha 2 and the Drosophila engrailed homeodomain-DNA complexes shows that the protein fold is highly conserved, despite a 3-residue insertion in alpha 2 and only 27% sequence identity between the two homeodomains. Moreover, the orientation of the recognition helix on the DNA is also conserved. This docking arrangement is maintained by side chain contacts with the DNA--primarily the sugar-phosphate backbone--that are identical in alpha 2 and engrailed. Since these residues are conserved among all homeodomains, we propose that the contacts with the DNA are also conserved and suggest a general model for homeodomain-DNA interactions.

PubMed: 1682054
DOI: 10.1016/0092-8674(91)90526-5

実験手法

X-RAY DIFFRACTION (2.7 Å)