1ANW

THE EFFECT OF METAL BINDING ON THE STRUCTURE OF ANNEXIN V AND IMPLICATIONS FOR MEMBRANE BINDING

1ANW の概要

• エントリーDOI: 10.2210/pdb1anw/pdb
• 分子名称: ANNEXIN V, CALCIUM ION (3 entities in total)
• 機能のキーワード: calcium/phospholipid-binding protein, calcium-phospholipid-binding protein complex
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 71859.34

構造登録者

Lewit-Bentley, A.,Morera, S.,Huber, R.,Bodo, G. (登録日: 1993-10-26, 公開日: 1994-12-20, 最終更新日: 2024-02-07)

主引用文献

Lewit-Bentley, A.,Morera, S.,Huber, R.,Bodo, G.
The effect of metal binding on the structure of annexin V and implications for membrane binding.
Eur.J.Biochem., 210:73-77, 1992

PubMed Abstract: The structure of annexin V, crystallised in the presence of two calcium or barium ions for each protein molecule, was solved by molecular replacement to 0.24 nm resolution. The two metal ions are found in domains I and IV, i.e. on the same side of the channel that lies in the centre of the molecule. The structures of the barium and calcium form are extremely close, the only differences localised in the metal-binding sites that lie on the surface of the molecule. The occupancies of the metal ions, however, are lower for barium than for calcium, expressing the lower affinity of the protein for the former. The packing of the annexin molecules in the crystal asymmetric unit may represent a model for the calcium driven association of membrane-bound annexins that leads to membrane fusion.

PubMed: 1446685
DOI: 10.1111/j.1432-1033.1992.tb17392.x

実験手法

X-RAY DIFFRACTION (2.4 Å)