1ANP

SOLUTION CONFORMATION OF AN ATRIAL NATRIURETIC PEPTIDE VARIANT SELECTIVE FOR THE TYPE-A RECEPTOR

1ANP の概要

• エントリーDOI: 10.2210/pdb1anp/pdb
• 分子名称: ATRIAL NATRIURETIC PEPTIDE (1 entity in total)
• 機能のキーワード: hypotensive hormone
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P01160
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3031.32

構造登録者

Fairbrother, W.J.,Mcdowell, R.S.,Cunningham, B.C. (登録日: 1994-04-06, 公開日: 1995-04-07, 最終更新日: 2024-04-10)

主引用文献

Fairbrother, W.J.,McDowell, R.S.,Cunningham, B.C.
Solution conformation of an atrial natriuretic peptide variant selective for the type A receptor.
Biochemistry, 33:8897-8904, 1994

PubMed Abstract: Two-dimensional NMR spectroscopy has been used to characterize the solution conformation of an atrial natriuretic peptide (ANP) variant which is selective for the human natriuretic peptide receptor A (NPR-A) relative to receptor C (NPR-C). The ANP mutant, containing six substitutions, has reduced flexibility in aqueous solution relative to wild-type ANP and allows the observation of sufficient NOE connectivities for structure determination by distance geometry and restrained molecular dynamics calculations. The solution conformation is reasonably well defined, having an average backbone atom rms deviation from the average coordinates of approximately 1.1 A for residues 7-27. The structure is consistent with available functional data and shows a spatial separation between known receptor binding determinants and residues found to be outside the hormone-receptor interface.

PubMed: 8043577
DOI: 10.1021/bi00196a006

実験手法

SOLUTION NMR