1AN1

LEECH-DERIVED TRYPTASE INHIBITOR/TRYPSIN COMPLEX

1AN1 の概要

• エントリーDOI: 10.2210/pdb1an1/pdb
• 分子名称: TRYPSIN, TRYPTASE INHIBITOR, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: serine proteinase inhibitor, tryptase inhibition, non-classical kazal-type inhibitor, complex (serine protease-inhibitor), complex (serine protease-inhibitor) complex, complex (serine protease/inhibitor)
• 由来する生物種: Sus scrofa (pig); 詳細
• 細胞内の位置: Secreted, extracellular space: P00761
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28285.17

構造登録者

Priestle, J.P.,Di Marco, S. (登録日: 1997-06-26, 公開日: 1998-07-01, 最終更新日: 2024-11-20)

主引用文献

Di Marco, S.,Priestle, J.P.
Structure of the complex of leech-derived tryptase inhibitor (LDTI) with trypsin and modeling of the LDTI-tryptase system.
Structure, 5:1465-1474, 1997

PubMed Abstract: Tryptase is a trypsin-like serine proteinase stored in the cytoplasmic granules of mast cells, which has been implicated in a number of mast cell related disorders such as asthma and rheumatoid arthritis. Unlike almost all other serine proteinases, tryptase is fully active in plasma and in the extracellular space, as there are no known natural inhibitors of tryptase in humans. Leech-derived tryptase inhibitor (LDTI), a protein of 46 amino acids, is the first molecule found to bind tightly to and specifically inhibit human tryptase in the nanomolar range. LDTI also inhibits trypsin and chymotrypsin with similar affinities. The structure of LDTI in complex with an inhibited proteinase could be used as a template for the development of low molecular weight tryptase inhibitors.

PubMed: 9384562
DOI: 10.1016/S0969-2126(97)00296-7

実験手法

X-RAY DIFFRACTION (2.03 Å)