1AJD

THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY

Summary for 1AJD

Entry DOI	10.2210/pdb1ajd/pdb
Descriptor	ALKALINE PHOSPHATASE INTERMEDIATE II OF HOLO ENZYME, ZINC ION, MAGNESIUM ION, ... (4 entities in total)
Functional Keywords	non specific mono-esterase
Biological source	Escherichia coli
Cellular location	Periplasm: P00634
Total number of polymer chains	2
Total formula weight	94384.94
Authors	Dealwis, C.G.,Chen, L.,Abad-Zapatero, C. (deposition date: 1995-08-18, release date: 1995-11-14, Last modification date: 2021-11-03)
Primary citation	Dealwis, C.G.,Brennan, C.,Christianson, K.,Mandecki, W.,Abad-Zapatero, C. Crystallographic analysis of reversible metal binding observed in a mutant (Asp153-->Gly) of Escherichia coli alkaline phosphatase. Biochemistry, 34:13967-13973, 1995 Cited by PubMed: 7577993 DOI: 10.1021/bi00043a001 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.5 Å)

Structure validation