1AIF

ANTI-IDIOTYPIC FAB 409.5.3 (IGG2A) FAB FROM MOUSE

1AIF の概要

• エントリーDOI: 10.2210/pdb1aif/pdb
• 分子名称: ANTI-IDIOTYPIC FAB 409.5.3 (IGG2A) FAB (LIGHT CHAIN), ANTI-IDIOTYPIC FAB 409.5.3 (IGG2A) FAB (HEAVY CHAIN) (2 entities in total)
• 機能のキーワード: immunoglobulin, c region, v region
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 94747.24

構造登録者

Ban, N.,Escobar, C.,Hasel, K.,Day, J.,Greenwood, A.,McPherson, A. (登録日: 1994-11-14, 公開日: 1997-02-01, 最終更新日: 2024-10-30)

主引用文献

Ban, N.,Escobar, C.,Hasel, K.W.,Day, J.,Greenwood, A.,McPherson, A.
Structure of an anti-idiotypic Fab against feline peritonitis virus-neutralizing antibody and a comparison with the complexed Fab.
FASEB J., 9:107-114, 1995

PubMed Abstract: The crystal structure of anti-idiotopic Fab 409.5.3, made against an E2 specific feline infectious peritonitis virus-neutralizing antibody 730.1.4, has been determined in its free from, at 2.9 A resolution by molecular replacement. This antibody, used as an immmunogen, elicits the production of anti-anti-idiotypic antibodies that in turn neutralize the virus. The structure of the uncomplexed Fab was refined using constrained-restrained least squares minimization and simulated annealing in combination with conjugate gradient techniques to a crystallographic R of 0.22 based on 16,482 unique reflections between 20.0 and 2.9 A. The free antiidiotypic Fab shows, when compared to its complexed form, a 5 degrees rotation of its variable light with respect to its variable heavy domain and rearrangement of complementarity determining region loops, which permits optimization of the stereocomplementarity between interacting molecules. This finding supports the induced fit hypothesis for antibody antigen interaction.

PubMed: 7821749

実験手法

X-RAY DIFFRACTION (2.9 Å)