1AG6

PLASTOCYANIN FROM SPINACH

1AG6 の概要

• エントリーDOI: 10.2210/pdb1ag6/pdb
• 分子名称: PLASTOCYANIN, COPPER (II) ION (3 entities in total)
• 機能のキーワード: blue-copper protein, electron transfer, electron transport
• 由来する生物種: Spinacia oleracea (spinach)
• 細胞内の位置: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side: P00289
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10542.14

構造登録者

Xue, Y.,Okvist, M.,Young, S. (登録日: 1997-04-02, 公開日: 1998-10-21, 最終更新日: 2024-05-22)

主引用文献

Xue, Y.,Okvist, M.,Hansson, O.,Young, S.
Crystal structure of spinach plastocyanin at 1.7 A resolution.
Protein Sci., 7:2099-2105, 1998

PubMed Abstract: The crystal structure of plastocyanin from spinach has been determined using molecular replacement, with the structure of plastocyanin from poplar as a search model. Successful crystallization was facilitated by site-directed mutagenesis in which residue Gly8 was substituted with Asp. The region around residue 8 was believed to be too mobile for the wild-type protein to form crystals despite extensive screening. The current structure represents the oxidized plastocyanin, copper (II), at low pH (approximately 4.4). In contrast to the similarity in the core region as compared to its poplar counterpart, the structure shows some significant differences in loop regions. The most notable is the large shift of the 59-61 loop where the largest shift is 3.0 A for the C(alpha) atom of Glu59. This results in different patterns of electrostatic potential around the acidic patches for the two proteins.

PubMed: 9792096

実験手法

X-RAY DIFFRACTION (1.6 Å)