1AFV

HIV-1 CAPSID PROTEIN (P24) COMPLEX WITH FAB25.3

Summary for 1AFV

• Entry DOI: 10.2210/pdb1afv/pdb
• Descriptor: HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 CAPSID PROTEIN, ANTIBODY FAB25.3 FRAGMENT (LIGHT CHAIN), ANTIBODY FAB25.3 FRAGMENT (HEAVY CHAIN), ... (4 entities in total)
• Functional Keywords: complex (viral capsid-immunoglobulin), hiv, capsid protein, p24, viral protein-immune system complex, viral protein/immune system
• Biological source: Human immunodeficiency virus 1; More
• Cellular location: Matrix protein p17: Virion (Potential). Capsid protein p24: Virion (Potential). Nucleocapsid protein p7: Virion (Potential). Reverse transcriptase/ribonuclease H: Virion (Potential). Integrase: Virion (Potential): P12497
• Total number of polymer chains: 6
• Total formula weight: 128732.14

Authors

Momany, C.,Kovari, L.C.,Prongay, A.J.,Keller, W.,Gitti, R.K.,Lee, B.M.,Gorbalenya, A.E.,Tong, L.,Mcclure, J.,Ehrlich, L.S.,Summers, M.F.,Carter, C.,Rossmann, M.G. (deposition date: 1997-03-14, release date: 1997-08-20, Last modification date: 2024-10-09)

Primary citation

Momany, C.,Kovari, L.C.,Prongay, A.J.,Keller, W.,Gitti, R.K.,Lee, B.M.,Gorbalenya, A.E.,Tong, L.,McClure, J.,Ehrlich, L.S.,Summers, M.F.,Carter, C.,Rossmann, M.G.
Crystal structure of dimeric HIV-1 capsid protein.
Nat.Struct.Biol., 3:763-770, 1996

PubMed Abstract: X-ray diffraction analysis of a human immunodeficiency virus (HIV-1) capsid (CA) protein shows that each monomer within the dimer consists of seven alpha-helices, five of which are arranged in a coiled coil-like structure. Sequence assignments were made for two of the helices, and tentative connectivity of the remainder of the protein was confirmed by the recent solution structure of a monomeric N-terminal fragment. The C-terminal third of the protein is mostly disordered in the crystal. The longest helices in the coiled coil-like structure are separated by a long, highly antigenic peptide that includes the binding site of an antibody fragment complexed with CA in the crystal. The site of binding of the Fab, the position of the antigenic loop and the site of cleavage between the matrix protein and CA establish the side of the dimer that would be on the exterior of the retroviral core.

PubMed: 8784350
DOI: 10.1038/nsb0996-763

Experimental method

X-RAY DIFFRACTION (3.7 Å)