1AFV

HIV-1 CAPSID PROTEIN (P24) COMPLEX WITH FAB25.3

1AFV の概要

• エントリーDOI: 10.2210/pdb1afv/pdb
• 分子名称: HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 CAPSID PROTEIN, ANTIBODY FAB25.3 FRAGMENT (LIGHT CHAIN), ANTIBODY FAB25.3 FRAGMENT (HEAVY CHAIN), ... (4 entities in total)
• 機能のキーワード: complex (viral capsid-immunoglobulin), hiv, capsid protein, p24, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• 細胞内の位置: Matrix protein p17: Virion (Potential). Capsid protein p24: Virion (Potential). Nucleocapsid protein p7: Virion (Potential). Reverse transcriptase/ribonuclease H: Virion (Potential). Integrase: Virion (Potential): P12497
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 128732.14

構造登録者

Momany, C.,Kovari, L.C.,Prongay, A.J.,Keller, W.,Gitti, R.K.,Lee, B.M.,Gorbalenya, A.E.,Tong, L.,Mcclure, J.,Ehrlich, L.S.,Summers, M.F.,Carter, C.,Rossmann, M.G. (登録日: 1997-03-14, 公開日: 1997-08-20, 最終更新日: 2024-10-09)

主引用文献

Momany, C.,Kovari, L.C.,Prongay, A.J.,Keller, W.,Gitti, R.K.,Lee, B.M.,Gorbalenya, A.E.,Tong, L.,McClure, J.,Ehrlich, L.S.,Summers, M.F.,Carter, C.,Rossmann, M.G.
Crystal structure of dimeric HIV-1 capsid protein.
Nat.Struct.Biol., 3:763-770, 1996

PubMed Abstract: X-ray diffraction analysis of a human immunodeficiency virus (HIV-1) capsid (CA) protein shows that each monomer within the dimer consists of seven alpha-helices, five of which are arranged in a coiled coil-like structure. Sequence assignments were made for two of the helices, and tentative connectivity of the remainder of the protein was confirmed by the recent solution structure of a monomeric N-terminal fragment. The C-terminal third of the protein is mostly disordered in the crystal. The longest helices in the coiled coil-like structure are separated by a long, highly antigenic peptide that includes the binding site of an antibody fragment complexed with CA in the crystal. The site of binding of the Fab, the position of the antigenic loop and the site of cleavage between the matrix protein and CA establish the side of the dimer that would be on the exterior of the retroviral core.

PubMed: 8784350
DOI: 10.1038/nsb0996-763

実験手法

X-RAY DIFFRACTION (3.7 Å)