1AF5

GROUP I MOBILE INTRON ENDONUCLEASE

1AF5 の概要

• エントリーDOI: 10.2210/pdb1af5/pdb
• 分子名称: I-CREI (2 entities in total)
• 機能のキーワード: endonuclease, group i mobile intron, intron homing, chloroplast dna, laglidadg motif
• 由来する生物種: Chlamydomonas reinhardtii
• 細胞内の位置: Plastid, chloroplast: P05725
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15551.93

構造登録者

Heath, P.J.,Stephens, K.M.,Monnat Junior, R.J.,Stoddard, B.L. (登録日: 1997-03-21, 公開日: 1997-07-23, 最終更新日: 2024-02-07)

主引用文献

Heath, P.J.,Stephens, K.M.,Monnat Jr., R.J.,Stoddard, B.L.
The structure of I-Crel, a group I intron-encoded homing endonuclease.
Nat.Struct.Biol., 4:468-476, 1997

PubMed Abstract: The structure of I-Crel provides the first view of a protein encoded by a gene within an intron. This endonuclease recognizes a long DNA site approximately 20 base pairs in length and facilitates the lateral transfer of that intron. The protein exhibits a DNA-binding surface consisting of four antiparallel beta-strands that form a 20 A wide groove which is over 70 A long. The architecture of this fold is different from that of the TATA binding protein, TBP, which also contains an antiparallel beta-saddle. The conserved LAGLIDADG motif, which is found in many mobile intron endonucleases, maturases and inteins, forms a novel helical interface and contributes essential residues to the active site.

PubMed: 9187655
DOI: 10.1038/nsb0697-468

実験手法

X-RAY DIFFRACTION (3 Å)