1ABY

CYANOMET RHB1.1 (RECOMBINANT HEMOGLOBIN)

1ABY の概要

• エントリーDOI: 10.2210/pdb1aby/pdb
• 分子名称: HEMOGLOBIN, CYANIDE ION, PROTOPORPHYRIN IX CONTAINING FE, ... (5 entities in total)
• 機能のキーワード: heme, oxygen transport, respiratory protein, erythrocyte
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 64816.55

構造登録者

Kundrot, C.E.,Kroeger, K.S. (登録日: 1997-01-29, 公開日: 1998-02-25, 最終更新日: 2024-04-03)

主引用文献

Kroeger, K.S.,Kundrot, C.E.
Structures of a hemoglobin-based blood substitute: insights into the function of allosteric proteins.
Structure, 5:227-237, 1997

PubMed Abstract: . Potential blood substitutes can be based on hemoglobin. Two problems must be overcome with acellular hemoglobin-based blood substitutes, however: the oxygen affinity of purified human hemoglobin is too high for it to deliver oxygen to tissues, and hemoglobin tetramers dissociate into alphabeta dimers that can cause kidney damage. A modified form of hemoglobin, rHb 1.1, has reduced oxygen affinity as the result of an Asnbeta 108-->Lys mutation, and dimerization is prevented by the insertion of a glycine residue between the sequences of the normal alpha chains to produce one covalently continuous di-alpha-chain. Determination of the structure of rHb 1.1 would provide structure-based explanations for the altered properties of rHb 1.1.

PubMed: 9032082
DOI: 10.1016/S0969-2126(97)00181-0

実験手法

X-RAY DIFFRACTION (2.6 Å)