1AAR

STRUCTURE OF A DIUBIQUITIN CONJUGATE AND A MODEL FOR INTERACTION WITH UBIQUITIN CONJUGATING ENZYME (E2)

1AAR の概要

• エントリーDOI: 10.2210/pdb1aar/pdb
• 分子名称: DI-UBIQUITIN (2 entities in total)
• 機能のキーワード: ubiquitin
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17153.66

構造登録者

Cook, W.J.,Jeffrey, L.C.,Carson, M.,Chen, Z.,Pickart, C.M. (登録日: 1992-04-17, 公開日: 1993-10-31, 最終更新日: 2024-10-23)

主引用文献

Cook, W.J.,Jeffrey, L.C.,Carson, M.,Chen, Z.,Pickart, C.M.
Structure of a diubiquitin conjugate and a model for interaction with ubiquitin conjugating enzyme (E2).
J.Biol.Chem., 267:16467-16471, 1992

PubMed Abstract: Covalent ligation of multiubiquitin chains targets eukaryotic proteins for degradation. In such multiubiquitin chains, successive ubiquitins are linked by an isopeptide bond involving the side chain of Lys48 and the carboxyl group of Gly76. The crystal structure of a diubiquitin conjugate has been determined and refined at 2.3-A resolution. The molecule has internal approximate 2-fold symmetry with multiple hydrophobic and hydrophilic contacts along the 2-fold axis. The structure of the diubiquitin conjugate suggests determinants for recognition of multiubiquitin chains. A model for the interaction of diubiquitin and a ubiquitin conjugating enzyme (E2) is proposed.

PubMed: 1322903

実験手法

X-RAY DIFFRACTION (2.3 Å)