1A70

SPINACH FERREDOXIN

1A70 の概要

• エントリーDOI: 10.2210/pdb1a70/pdb
• 分子名称: FERREDOXIN, FE2/S2 (INORGANIC) CLUSTER (3 entities in total)
• 機能のキーワード: iron-sulfur protein, photosynthesis, electron transport
• 由来する生物種: Spinacia oleracea (spinach)
• 細胞内の位置: Plastid, chloroplast: P00221
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10664.29

構造登録者

Binda, C.,Coda, A.,Mattevi, A.,Aliverti, A.,Zanetti, G. (登録日: 1998-03-19, 公開日: 1998-11-25, 最終更新日: 2024-05-22)

主引用文献

Binda, C.,Coda, A.,Aliverti, A.,Zanetti, G.,Mattevi, A.
Structure of the mutant E92K of [2Fe-2S] ferredoxin I from Spinacia oleracea at 1.7 A resolution.
Acta Crystallogr.,Sect.D, 54:1353-1358, 1998

PubMed Abstract: Ferredoxin I (Fd I) from Spinacia oleracea is composed of 97 amino-acid residues and a [2Fe-2S] cluster. The crystal structure of the E92K mutant of Fd I was solved by molecular replacement and refined to an R factor of 19.6% for 11755 reflections at 1.7 A resolution. The overall structure and the active centre of spinach Fd is highly conserved with respect to ferredoxins of known structure. The E92K mutation appears to disturb a hydrogen-bond network which stabilizes the loop bearing the [2Fe-2S] cluster. This observation provides a rationale for the reduced electron-transfer efficiency displayed by the E92K mutant. Inspection of the crystal packing reveals that the side chain of Lys92 is engaged in an intermolecular interaction with Asp26 of a symmetry-related molecule. This feature may explain why only the mutant E92K and not wild-type Fd I could be successfully crystallized.

PubMed: 10089511
DOI: 10.1107/S0907444998005137

実験手法

X-RAY DIFFRACTION (1.7 Å)