1A3O

ARTIFICIAL MUTANT (ALPHA Y42H) OF DEOXY HEMOGLOBIN

1A3O の概要

• エントリーDOI: 10.2210/pdb1a3o/pdb
• 分子名称: HEMOGLOBIN (ALPHA CHAIN), HEMOGLOBIN (BETA CHAIN), PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• 機能のキーワード: oxygen transport, heme, respiratory protein, erythrocyte
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64497.00

構造登録者

Tame, J.,Vallone, B. (登録日: 1998-01-22, 公開日: 1998-04-29, 最終更新日: 2024-02-07)

主引用文献

Tame, J.R.,Vallone, B.
The structures of deoxy human haemoglobin and the mutant Hb Tyralpha42His at 120 K.
Acta Crystallogr.,Sect.D, 56:805-811, 2000

PubMed Abstract: The structures of deoxy human haemoglobin and an artificial mutant (Tyralpha42-->His) have been solved at 120 K. While overall agreement between these structures and others in the PDB is very good, certain side chains are found to be shifted, absent from the electron-density map or in different rotamers. Non-crystallographic symmetry (NCS) is very well obeyed in the native protein, but not around the site of the changed residue in the mutant. NCS is also not obeyed by the water molecule invariably found in the alpha-chain haem pocket in room-temperature crystal structures of haemoglobin. At 120 K, this water molecule disappears from one alpha chain in the asymmetric unit but not the other.

PubMed: 10930827
DOI: 10.1107/S0907444900006387

実験手法

X-RAY DIFFRACTION (1.8 Å)