1A29

CALMODULIN COMPLEXED WITH TRIFLUOPERAZINE (1:2 COMPLEX)

1A29 の概要

• エントリーDOI: 10.2210/pdb1a29/pdb
• 分子名称: CALMODULIN, CALCIUM ION, 10-[3-(4-METHYL-PIPERAZIN-1-YL)-PROPYL]-2-TRIFLUOROMETHYL-10H-PHENOTHIAZINE (3 entities in total)
• 機能のキーワード: calcium-binding protein
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Cytoplasm: P62157
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17696.65

構造登録者

Bocskei, Zs.,Harmat, V.,Vertessy, B.G.,Ovadi, J.,Naray-Szabo, G. (登録日: 1998-01-19, 公開日: 1998-09-16, 最終更新日: 2024-05-22)

主引用文献

Vertessy, B.G.,Harmat, V.,Bocskei, Z.,Naray-Szabo, G.,Orosz, F.,Ovadi, J.
Simultaneous binding of drugs with different chemical structures to Ca2+-calmodulin: crystallographic and spectroscopic studies.
Biochemistry, 37:15300-15310, 1998

PubMed Abstract: The modulatory action of Ca2+-calmodulin on multiple targets is inhibited by trifluoperazine, which competes with target proteins for calmodulin binding. The structure of calmodulin crystallized with two trifluoperazine molecules is determined by X-ray crystallography at 2.74 A resolution. The X-ray data together with the characteristic and distinct signals obtained by circular dichroism in solution allowed us to identify the binding domains as well as the order of the binding of two trifluoperazine molecules to calmodulin. Accordingly, the binding of trifluperazine to the C-terminal hydrophobic pocket is followed by the interaction of the second drug molecule with an interdomain site. Recently, we demonstrated that the two bisindole derivatives, vinblastine and KAR-2 [3"-(beta-chloroethyl)-2",4"-dioxo-3, 5"-spirooxazolidino-4-deacetoxyvinblastine], interact with calmodulin with comparable affinity; however, they display different functional effects [Orosz et al. (1997) British J. Pharmacol. 121, 955-962]. The structural basis responsible for these effects were investigated by circular dichroism and fluorescence spectroscopy. The data provide evidence that calmodulin can simultaneously accommodate trifluoperazine and KAR-2 as well as vinblastine and KAR-2, but not trifluoperazine and vinblastine. The combination of the binding and structural data suggests that distinct binding sites exist on calmodulin for vinblastine and KAR-2 which correspond, at least partly, to that of trifluoperazine at the C-terminal hydrophobic pocket and at an interdomain site, respectively. This structural arrangement can explain why these drugs display different anticalmodulin activities. Calmodulin complexed with melittin is also able to bind two trifluoperazine molecules, the binding of which appears to be cooperative. Results obtained with intact and proteolytically cleaved calmodulin reveal that the central linker region of the protein is indispensable for simultanous interactions with two molecules of either identical or different ligands.

PubMed: 9799490
DOI: 10.1021/bi980795a

実験手法

X-RAY DIFFRACTION (2.74 Å)