1A17

TETRATRICOPEPTIDE REPEATS OF PROTEIN PHOSPHATASE 5

1A17 の概要

• エントリーDOI: 10.2210/pdb1a17/pdb
• 分子名称: SERINE/THREONINE PROTEIN PHOSPHATASE 5, SULFATE ION (3 entities in total)
• 機能のキーワード: hydrolase, phosphatase, protein-protein interactions, tpr, super-helix
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus : P53041
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19252.67

構造登録者

Das, A.K.,Cohen, P.T.W.,Barford, D. (登録日: 1997-12-23, 公開日: 1998-04-29, 最終更新日: 2024-02-07)

主引用文献

Das, A.K.,Cohen, P.W.,Barford, D.
The structure of the tetratricopeptide repeats of protein phosphatase 5: implications for TPR-mediated protein-protein interactions.
EMBO J., 17:1192-1199, 1998

PubMed Abstract: The tetratricopeptide repeat (TPR) is a degenerate 34 amino acid sequence identified in a wide variety of proteins, present in tandem arrays of 3-16 motifs, which form scaffolds to mediate protein-protein interactions and often the assembly of multiprotein complexes. TPR-containing proteins include the anaphase promoting complex (APC) subunits cdc16, cdc23 and cdc27, the NADPH oxidase subunit p67 phox, hsp90-binding immunophilins, transcription factors, the PKR protein kinase inhibitor, and peroxisomal and mitochondrial import proteins. Here, we report the crystal structure of the TPR domain of a protein phosphatase, PP5. Each of the three TPR motifs of this domain consist of a pair of antiparallel alpha-helices of equivalent length. Adjacent TPR motifs are packed together in a parallel arrangement such that a tandem TPR motif structure is composed of a regular series of antiparallel alpha-helices. The uniform angular and spatial arrangement of neighbouring alpha-helices defines a helical structure and creates an amphipathic groove. Multiple-TPR motif proteins would fold into a right-handed super-helical structure with a continuous helical groove suitable for the recognition of target proteins, hence defining a novel mechanism for protein recognition. The spatial arrangement of alpha-helices in the PP5-TPR domain is similar to those within 14-3-3 proteins.

PubMed: 9482716
DOI: 10.1093/emboj/17.5.1192

実験手法

X-RAY DIFFRACTION (2.45 Å)