1A15

SDF-1ALPHA

1A15 の概要

• エントリーDOI: 10.2210/pdb1a15/pdb
• 分子名称: STROMAL DERIVED FACTOR-1ALPHA, SULFATE ION (3 entities in total)
• 機能のキーワード: chemokine, human stromal cell-derived factor-1alpha
• 細胞内の位置: Secreted: P48061
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15708.57

構造登録者

Dealwis, C.G.,Fernandez, E.J.,Lolis, E. (登録日: 1997-12-22, 公開日: 1998-08-12, 最終更新日: 2024-10-23)

主引用文献

Dealwis, C.,Fernandez, E.J.,Thompson, D.A.,Simon, R.J.,Siani, M.A.,Lolis, E.
Crystal structure of chemically synthesized [N33A] stromal cell-derived factor 1alpha, a potent ligand for the HIV-1 "fusin" coreceptor.
Proc.Natl.Acad.Sci.USA, 95:6941-6946, 1998

PubMed Abstract: Stromal cell-derived factor-1alpha (SDF-1alpha ) is a member of the chemokine superfamily and functions as a growth factor and chemoattractant through activation of CXCR4/LESTR/Fusin, a G protein-coupled receptor. This receptor also functions as a coreceptor for T-tropic syncytium-inducing strains of HIV-1. SDF-1alpha antagonizes infectivity of these strains by competing with gp120 for binding to the receptor. The crystal structure of a variant SDF-1alpha ([N33A]SDF-1alpha ) prepared by total chemical synthesis has been refined to 2.2-A resolution. Although SDF-1alpha adopts a typical chemokine beta-beta-beta-alpha topology, the packing of the alpha-helix against the beta-sheet is strikingly different. Comparison of SDF-1alpha with other chemokine structures confirms the hypothesis that SDF-1alpha may be either an ancestral protein from which all other chemokines evolved or the chemokine that is the least divergent from a primordial chemokine. The structure of SDF-1alpha reveals a positively charged surface ideal for binding to the negatively charged extracellular loops of the CXCR4 HIV-1 coreceptor. This ionic complementarity is likely to promote the interaction of the mobile N-terminal segment of SDF-1alpha with interhelical sites of the receptor, resulting in a biological response.

PubMed: 9618518
DOI: 10.1073/pnas.95.12.6941

実験手法

X-RAY DIFFRACTION (2.2 Å)