1A0A

PHOSPHATE SYSTEM POSITIVE REGULATORY PROTEIN PHO4/DNA COMPLEX

Summary for 1A0A

• Entry DOI: 10.2210/pdb1a0a/pdb
• Descriptor: DNA (5'-D(*CP*TP*CP*AP*CP*AP*CP*GP*TP*GP*GP*GP*AP*CP*TP*AP*G )-3'), DNA (5'-D(*CP*TP*AP*GP*TP*CP*CP*CP*AP*CP*GP*TP*GP*TP*GP*AP*G )-3'), PROTEIN (PHOSPHATE SYSTEM POSITIVE REGULATORY PROTEIN PHO4), ... (4 entities in total)
• Functional Keywords: transcription factor, basic helix loop helix, complex (transcription factor-dna), transcription-dna complex, transcription/dna
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Cellular location: Cytoplasm : P07270
• Total number of polymer chains: 4
• Total formula weight: 24623.88

Authors

Shimizu, T.,Toumoto, A.,Ihara, K.,Shimizu, M.,Kyogoku, Y.,Ogawa, N.,Oshima, Y.,Hakoshima, T. (deposition date: 1997-11-27, release date: 1998-03-18, Last modification date: 2024-02-07)

Primary citation

Shimizu, T.,Toumoto, A.,Ihara, K.,Shimizu, M.,Kyogoku, Y.,Ogawa, N.,Oshima, Y.,Hakoshima, T.
Crystal structure of PHO4 bHLH domain-DNA complex: flanking base recognition.
EMBO J., 16:4689-4697, 1997

PubMed Abstract: The crystal structure of a DNA-binding domain of PHO4 complexed with DNA at 2.8 A resolution revealed that the domain folds into a basic-helix-loop-helix (bHLH) motif with a long but compact loop that contains a short alpha-helical segment. This helical structure positions a tryptophan residue into an aromatic cluster so as to make the loop compact. PHO4 binds to DNA as a homodimer with direct reading of both the core E-box sequence CACGTG and its 3'-flanking bases. The 3'-flanking bases GG are recognized by Arg2 and His5. The residues involved in the E-box recognition are His5, Glu9 and Arg13, as already reported for bHLH/Zip proteins MAX and USF, and are different from those recognized by bHLH proteins MyoD and E47, although PHO4 is a bHLH protein.

PubMed: 9303313
DOI: 10.1093/emboj/16.15.4689

Experimental method

X-RAY DIFFRACTION (2.8 Å)