1A0A

PHOSPHATE SYSTEM POSITIVE REGULATORY PROTEIN PHO4/DNA COMPLEX

1A0A の概要

• エントリーDOI: 10.2210/pdb1a0a/pdb
• 分子名称: DNA (5'-D(*CP*TP*CP*AP*CP*AP*CP*GP*TP*GP*GP*GP*AP*CP*TP*AP*G )-3'), DNA (5'-D(*CP*TP*AP*GP*TP*CP*CP*CP*AP*CP*GP*TP*GP*TP*GP*AP*G )-3'), PROTEIN (PHOSPHATE SYSTEM POSITIVE REGULATORY PROTEIN PHO4), ... (4 entities in total)
• 機能のキーワード: transcription factor, basic helix loop helix, complex (transcription factor-dna), transcription-dna complex, transcription/dna
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Cytoplasm : P07270
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 24623.88

構造登録者

Shimizu, T.,Toumoto, A.,Ihara, K.,Shimizu, M.,Kyogoku, Y.,Ogawa, N.,Oshima, Y.,Hakoshima, T. (登録日: 1997-11-27, 公開日: 1998-03-18, 最終更新日: 2024-02-07)

主引用文献

Shimizu, T.,Toumoto, A.,Ihara, K.,Shimizu, M.,Kyogoku, Y.,Ogawa, N.,Oshima, Y.,Hakoshima, T.
Crystal structure of PHO4 bHLH domain-DNA complex: flanking base recognition.
EMBO J., 16:4689-4697, 1997

PubMed Abstract: The crystal structure of a DNA-binding domain of PHO4 complexed with DNA at 2.8 A resolution revealed that the domain folds into a basic-helix-loop-helix (bHLH) motif with a long but compact loop that contains a short alpha-helical segment. This helical structure positions a tryptophan residue into an aromatic cluster so as to make the loop compact. PHO4 binds to DNA as a homodimer with direct reading of both the core E-box sequence CACGTG and its 3'-flanking bases. The 3'-flanking bases GG are recognized by Arg2 and His5. The residues involved in the E-box recognition are His5, Glu9 and Arg13, as already reported for bHLH/Zip proteins MAX and USF, and are different from those recognized by bHLH proteins MyoD and E47, although PHO4 is a bHLH protein.

PubMed: 9303313
DOI: 10.1093/emboj/16.15.4689

実験手法

X-RAY DIFFRACTION (2.8 Å)