1A06

CALMODULIN-DEPENDENT PROTEIN KINASE FROM RAT

1A06 の概要

• エントリーDOI: 10.2210/pdb1a06/pdb
• 分子名称: CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE (2 entities in total)
• 機能のキーワード: kinase, signal transduction, calcium/calmodulin
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Cytoplasm: Q63450
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37517.70

構造登録者

Kuriyan, J.,Goldberg, J. (登録日: 1997-12-09, 公開日: 1998-04-08, 最終更新日: 2024-02-07)

主引用文献

Goldberg, J.,Nairn, A.C.,Kuriyan, J.
Structural basis for the autoinhibition of calcium/calmodulin-dependent protein kinase I.
Cell(Cambridge,Mass.), 84:875-887, 1996

PubMed Abstract: The crystal structure of calcium/calmodulin-dependent protein kinase I has been determined in the autoinhibited form. The C-terminal regulatory region of the enzyme forms a helix-loop-helix segment that extends across the two domains of the catalytic core, making multiple inhibitory interactions. Elements of the first regulatory alpha helix and the loop interfere with the binding site for peptide substrates, while the loop and the second helix interact with the ATP-binding domain to induce conformational changes that obstruct the nucleotide binding pocket. One part of the calmodulin recognition element protrudes away from the catalytic domain and is potentially available for an initial interaction with calmodulin. The structure provides a view of an intact calmodulin target and suggests that substantial structural changes will accompany kinase activation by calmodulin binding to the regulatory region.

PubMed: 8601311
DOI: 10.1016/S0092-8674(00)81066-1

実験手法

X-RAY DIFFRACTION (2.5 Å)