1XYW

elk prion protein

Summary for 1XYW

• Entry DOI: 10.2210/pdb1xyw/pdb
• NMR Information: BMRB: 6383
• Descriptor: Major prion protein (1 entity in total)
• Functional Keywords: prp, cwd, prion protein, tse, unknown function
• Biological source: Cervus elaphus nelsoni (American elk)
• Cellular location: Cell membrane; Lipid-anchor, GPI-anchor (By similarity): P67986
• Total number of polymer chains: 1
• Total formula weight: 13120.57

Authors

Gossert, A.D.,Bonjour, S.,Lysek, D.A.,Fiorito, F.,Wuthrich, K. (deposition date: 2004-11-11, release date: 2004-12-28, Last modification date: 2024-10-16)

Primary citation

Gossert, A.D.,Bonjour, S.,Lysek, D.A.,Fiorito, F.,Wuthrich, K.
Prion protein NMR structures of elk and of mouse/elk hybrids
Proc.Natl.Acad.Sci.Usa, 102:646-650, 2005

PubMed Abstract: The NMR structure of the recombinant elk prion protein (ePrP), which represents the cellular isoform (ePrPC) in the healthy organism, is described here. As anticipated from the highly conserved amino acid sequence, ePrPC has the same global fold as other mammalian prion proteins (PrPs), with a flexibly disordered "tail" of residues 23-124 and a globular domain 125-226 with three alpha-helices and a short antiparallel beta-sheet. However, ePrPC shows a striking local structure variation when compared with most other mammalian PrPs, in particular human, bovine, and mouse PrPC. A loop of residues 166-175, which links the beta-sheet with the alpha2-helix and is part of a hypothetical "protein X" epitope, is outstandingly well defined, whereas this loop is disordered in the other species. Based on NMR structure determinations of two mouse PrP variants, mPrP[N174T] and mPrP[S170N,N174T], this study shows that the structured loop in ePrPC relates to these two local amino acid exchanges, so that mPrP[S170N,N174T] exactly mimics ePrPC. These results are evaluated in the context of recent reports on chronic wasting disease (CWD) in captive and free-ranging deer and elk in the U.S. and Canada, and an animal model is proposed for support of future research on CWD.

PubMed: 15647363
DOI: 10.1073/pnas.0409008102

Experimental method

SOLUTION NMR