1XO8

Solution structure of AT1g01470 from Arabidopsis Thaliana

Summary for 1XO8

• Entry DOI: 10.2210/pdb1xo8/pdb
• NMR Information: BMRB: 6339
• Descriptor: At1g01470 (1 entity in total)
• Functional Keywords: structural genomics, protein structure initiative, center for eukaryotic structural genomics, psi, cesg, unknown function
• Biological source: Arabidopsis thaliana (thale cress)
• Total number of polymer chains: 1
• Total formula weight: 16560.99

Authors

Singh, S.,Cornilescu, C.C.,Tyler, R.C.,Cornilescu, G.,Tonelli, M.,Lee, M.S.,Markley, J.L.,Center for Eukaryotic Structural Genomics (CESG) (deposition date: 2004-10-06, release date: 2004-10-19, Last modification date: 2024-05-22)

Primary citation

Singh, S.,Cornilescu, C.C.,Tyler, R.C.,Cornilescu, G.,Tonelli, M.,Lee, M.S.,Markley, J.L.
Solution structure of a late embryogenesis abundant protein (LEA14) from Arabidopsis thaliana, a cellular stress-related protein
Protein Sci., 14:2601-2609, 2005

PubMed Abstract: We report the three-dimensional structure of a late embryogenesis abundant (LEA) protein from Arabidopsis thaliana gene At1g01470.1. This protein is a member of Pfam cluster PF03168, and has been classified as a LEA14 protein. LEA proteins are expressed under conditions of cellular stress, such as desiccation, cold, osmotic stress, and heat. The structure, which was determined by NMR spectroscopy, revealed that the At1g01470.1 protein has an alphabeta-fold consisting of one alpha-helix and seven beta-strands that form two antiparallel beta-sheets. The closest structural homologs were discovered to be fibronectin Type III domains, which have <7% sequence identity. Because fibronectins from animal cells have been shown to be involved in cell adhesion, cell motility, wound healing, and maintenance of cell shape, it is interesting to note that in plants wounding or stress results in the overexpression of a protein with fibronectin Type III structural features.

PubMed: 16155204
DOI: 10.1110/ps.051579205

Experimental method

SOLUTION NMR