1XI1

Phi29 DNA polymerase ssDNA complex, monoclinic crystal form

Summary for 1XI1

• Entry DOI: 10.2210/pdb1xi1/pdb
• Related: 1XHX 1XHZ
• Descriptor: 5'-D(P*TP*TP*TP*TP*T)-3', DNA polymerase, MAGNESIUM ION, ... (4 entities in total)
• Functional Keywords: dna polymerase, protein-primed, strand displacement, processivity, replication, transferase-dna complex, transferase/dna
• Biological source: Bacillus phage phi29
• Total number of polymer chains: 4
• Total formula weight: 136442.45

Authors

Kamtekar, S.,Berman, A.J.,Wang, J.,Lazaro, J.M.,de Vega, M.,Blanco, L.,Salas, M.,Steitz, T.A. (deposition date: 2004-09-21, release date: 2004-12-07, Last modification date: 2024-02-14)

Primary citation

Wang, J.,Kamtekar, S.,Berman, A.J.,Steitz, T.A.
Correction of X-ray intensities from single crystals containing lattice-translocation defects
Acta Crystallogr.,Sect.D, 61:67-74, 2005

PubMed Abstract: In 1954, Howells and colleagues described an unusual diffraction pattern from imidazole methemoglobin crystals caused by lattice-translocation defects. In these crystals, two identical lattices coexist as a single coherent mosaic block, but are translated by a fixed vector with respect to each other. The observed structure is a weighted sum of the two identical but translated structures, one from each lattice; the observed structure factors are a weighted vector sum of the two structure factors with identical unit amplitudes but shifted phases. A general procedure is described to obtain the unit amplitudes of observed structure factors from a realigned single lattice through an X-ray intensity correction. An application of this procedure is made to determine the crystal structure of phi29 DNA polymerase at 2.2 A resolution using multiple isomorphous replacement and multiwavelength anomalous dispersion methods.

PubMed: 15608377
DOI: 10.1107/S0907444904026721

Experimental method

X-RAY DIFFRACTION (2.2 Å)