1X22
Solution structure of a novel moricin analogue, an antibacterial peptide from a lepidopteran insect, Spodoptera litura
Summary for 1X22
| Entry DOI | 10.2210/pdb1x22/pdb |
| Descriptor | moricin (1 entity in total) |
| Functional Keywords | insect immunity, antibacterial peptide, moricin, purification, gene expression, solution structure, antibiotic |
| Biological source | Spodoptera litura |
| Total number of polymer chains | 1 |
| Total formula weight | 4502.46 |
| Authors | Oizumi, Y.,Hemmi, H.,Minami, M.,Asaoka, A.,Yamakawa, M. (deposition date: 2005-04-18, release date: 2005-09-06, Last modification date: 2024-05-01) |
| Primary citation | Oizumi, Y.,Hemmi, H.,Minami, M.,Asaoka, A.,Yamakawa, M. Isolation, gene expression and solution structure of a novel moricin analogue, antibacterial peptide from a lepidopteran insect, Spodoptera litura Biochim.Biophys.Acta, 1752:83-92, 2005 Cited by PubMed Abstract: An antibacterial peptide was isolated from a lepidopteran insect, Spodoptera litura. The molecular mass of this peptide was determined to be 4489.55 by matrix assisted laser desorption/ionization-time of flight mass (MALDI-TOF MS) spectrometry. The peptide consists of 42 amino acids and the sequence has 69-98% identity to those of moricin-related peptides, antibacterial peptides from lepidopetran insects. Thus, the peptide was designated S. litura (Sl) moricin. Sl moricin showed a broad antibacterial spectrum against Gram-positive and negative bacteria. Sl moricin gene was inducible by bacterial injection and expressed tissue-specifically in the fat body and hemocytes. Furthermore, the solution structure of Sl moricin was determined by two-dimensional (2D) 1H-nuclear magnetic resonance (NMR) spectroscopy and hybrid distance geometry-simulated annealing calculation. The tertiary structure revealed a long alpha-helix containing eight turns along nearly the full length of the peptide like that of moricin, confirming that Sl moricin is a new moricin-like antibacterial peptide. These results suggest that moricin is present not only in B. mori but also in other lepidopteran insects forming a gene family. PubMed: 16115804DOI: 10.1016/j.bbapap.2005.07.013 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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