Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

4LEL

Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCG-G on the Ribosome

This is a non-PDB format compatible entry.
Summary for 4LEL
Entry DOI10.2210/pdb4lel/pdb
Descriptor16S rRNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (59 entities in total)
Functional Keywordsprotein biosynthesis, ribosomes, rna, trna, transfer rna, 30s, 70s, 16s, 23s, ribosomal subunit, ribosome
Biological sourceThermus thermophilus
More
Total number of polymer chains112
Total formula weight4435746.02
Authors
Maehigashi, T.,Dunkle, J.A.,Dunham, C.M. (deposition date: 2013-06-25, release date: 2014-08-06, Last modification date: 2023-09-20)
Primary citationMaehigashi, T.,Dunkle, J.A.,Miles, S.J.,Dunham, C.M.
Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.
Proc.Natl.Acad.Sci.USA, 111:12740-12745, 2014
Cited by
PubMed Abstract: Maintenance of the correct reading frame on the ribosome is essential for accurate protein synthesis. Here, we report structures of the 70S ribosome bound to frameshift suppressor tRNA(SufA6) and N1-methylguanosine at position 37 (m(1)G37) modification-deficient anticodon stem loop(Pro), both of which cause the ribosome to decode 4 rather than 3 nucleotides, resulting in a +1 reading frame. Our results reveal that decoding at +1 suppressible codons causes suppressor tRNA(SufA6) to undergo a rearrangement of its 5' stem that destabilizes U32, thereby disrupting the conserved U32-A38 base pair. Unexpectedly, the removal of the m(1)G37 modification of tRNA(Pro) also disrupts U32-A38 pairing in a structurally analogous manner. The lack of U32-A38 pairing provides a structural correlation between the transition from canonical translation and a +1 reading of the mRNA. Our structures clarify the molecular mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role played by the ribosome in maintaining the correct translational reading frame.
PubMed: 25128388
DOI: 10.1073/pnas.1409436111
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (3.90000024172 Å)
Structure validation

227344

PDB entries from 2024-11-13

PDB statisticsPDBj update infoContact PDBjnumon