1UXB

ADENOVIRUS AD19p FIBRE HEAD in complex with sialyl-lactose

Summary for 1UXB

• Entry DOI: 10.2210/pdb1uxb/pdb
• Related: 1UXA 1UXE
• Descriptor: FIBER PROTEIN, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose, N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose, ... (6 entities in total)
• Functional Keywords: viral protein, adenovirus, ad19p, fibre, receptor, sialic acid, neuraminic acid, cd46, daf, conjunctivitis
• Biological source: HUMAN ADENOVIRUS TYPE 19
• Total number of polymer chains: 3
• Total formula weight: 66939.96

Authors

Burmeister, W.P.,Guilligay, D.,Cusack, S.,Wadell, G.,Arnberg, N. (deposition date: 2004-02-24, release date: 2004-07-01, Last modification date: 2023-12-13)

Primary citation

Burmeister, W.P.,Guilligay, D.,Cusack, S.,Wadell, G.,Arnberg, N.
Crystal Structure of Species D Adenovirus Fiber Knobs and Their Sialic Acid Binding Sites
J.Virol., 78:7727-, 2004

PubMed Abstract: Adenovirus serotype 37 (Ad37) belongs to species D and can cause epidemic keratoconjunctivitis, whereas the closely related Ad19p does not. Primary cell attachment by adenoviruses is mediated through receptor binding of the knob domain of the fiber protein. The knobs of Ad37 and Ad19p differ at only two positions, Lys240Glu and Asn340Asp. We report the high-resolution crystal structures of the Ad37 and Ad19p knobs, both native and in complex with sialic acid, which has been proposed as a receptor for Ad37. Overall, the Ad37 and Ad19p knobs are very similar to previously reported knob structures, especially to that of Ad5, which binds the coxsackievirus-adenovirus receptor (CAR). Ad37 and Ad19p knobs are structurally identical with the exception of the changed side chains and are structurally most similar to CAR-binding knobs (e.g., that of Ad5) rather than non-CAR-binding knobs (e.g., that of Ad3). The two mutations in Ad19p result in a partial loss of the exceptionally high positive surface charge of the Ad37 knob but do not affect sialic acid binding. This site is located on the top of the trimer and binds both alpha(2,3) and alpha(2,6)-linked sialyl-lactose, although only the sialic acid residue makes direct contact. Amino acid alignment suggests that the sialic acid binding site is conserved in several species D serotypes. Our results show that the altered viral tropism and cell binding of Ad19p relative to those of Ad37 are not explained by a different binding ability toward sialyl-lactose.

PubMed: 15220447
DOI: 10.1128/JVI.78.14.7727-7736.2004

Experimental method

X-RAY DIFFRACTION (1.75 Å)