1UBI

SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1

Summary for 1UBI

• Entry DOI: 10.2210/pdb1ubi/pdb
• Descriptor: UBIQUITIN (2 entities in total)
• Functional Keywords: chromosomal protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 8576.83

Authors

Alexeev, D.,Bury, S.M.,Turner, M.A.,Ogunjobi, O.M.,Muir, T.W.,Ramage, R.,Sawyer, L. (deposition date: 1994-02-03, release date: 1994-05-31, Last modification date: 2024-02-14)

Primary citation

Ramage, R.,Green, J.,Muir, T.W.,Ogunjobi, O.M.,Love, S.,Shaw, K.
Synthetic, structural and biological studies of the ubiquitin system: the total chemical synthesis of ubiquitin.
Biochem.J., 299:151-158, 1994

PubMed Abstract: The small protein ubiquitin (76 amino acids) has been synthesized under optimized conditions by Merrifield solid-phase methodology using the N alpha-Fmoc protecting group. The crude polypeptide mixture was purified to homogeneity by gel filtration, dialysis and a combination of cation- and anion-exchange chromatography to yield ubiquitin. Amino acid analysis, enzymic digestion and sequencing by automated Edman degradation were used to authenticate the primary structure. Isoelectric focusing and m.s. were used to demonstrate that the final product was greater than 98% pure with a final yield of 93 mg (4.3%) from a single synthesis on a 0.25 nmol scale.

PubMed: 8166633

Experimental method

X-RAY DIFFRACTION (1.8 Å)