1RCB

CRYSTAL STRUCTURE OF HUMAN RECOMBINANT INTERLEUKIN-4 AT 2.25 ANGSTROMS RESOLUTION

Summary for 1RCB

• Entry DOI: 10.2210/pdb1rcb/pdb
• Descriptor: INTERLEUKIN-4 (2 entities in total)
• Functional Keywords: cytokine
• Biological source: Homo sapiens (human)
• Cellular location: Secreted: P05112
• Total number of polymer chains: 1
• Total formula weight: 14989.25

Authors

Wlodawer, A.,Pavlovsky, A.,Gustchina, A. (deposition date: 1992-08-26, release date: 1993-10-31, Last modification date: 2024-10-23)

Primary citation

Wlodawer, A.,Pavlovsky, A.,Gustchina, A.
Crystal structure of human recombinant interleukin-4 at 2.25 A resolution.
FEBS Lett., 309:59-64, 1992

PubMed Abstract: The crystal structure of human recombinant interleukin-4 (IL-4) has been solved by multiple isomorphous replacement, and refined to an R factor of 0.218 at 2.25 A resolution. The molecule is a left-handed four-helix bundle with a short stretch of beta sheet. The structure bears close resemblance to other cytokines such as granulocyte-macrophage colony stimulating factor (GM-CSF). Although no sequence similarity of IL-4 to GM-CSF and other related cytokines has been previously postulated, structure-based alignment of IL-4 and GM-CSF revealed that the core of the molecules, including large parts of all four helices and extending over half of the molecule, has 30% sequence identity. This may have identified regions which are not only important to maintain structure, but could also play a role in receptor binding.

PubMed: 1511746
DOI: 10.1016/0014-5793(92)80739-4

Experimental method

X-RAY DIFFRACTION (2.25 Å)