1RCA

STRUCTURE OF THE CRYSTALLINE COMPLEX OF DEOXYCYTIDYLYL-3',5'-GUANOSINE (3',5'-DCPDG) CO-CRYSTALISED WITH RIBONUCLEASE AT 1.9 ANGSTROMS RESOLUTION. RETROBINDING IN PANCREATIC RNASEA IS INDEPENDENT OF MODE OF INHIBITOR INTROMISSION

Summary for 1RCA

• Entry DOI: 10.2210/pdb1rca/pdb
• Descriptor: RIBONUCLEASE A, PHOSPHATE ION, 2'-DEOXYCYTIDINE-2'-DEOXYGUANOSINE-3',5'-MONOPHOSPHATE, ... (4 entities in total)
• Functional Keywords: hydrolase (endoribonuclease)
• Biological source: Bos taurus (cattle)
• Cellular location: Secreted: P61823
• Total number of polymer chains: 1
• Total formula weight: 14916.14

Authors

Lisgarten, J.N.,Palmer, R.A. (deposition date: 1994-07-18, release date: 1995-09-15, Last modification date: 2024-10-16)

Primary citation

Listgarten, J.N.,Maes, D.,Wyns, L.,Aguilar, C.F.,Palmer, R.A.
Structure of the crystalline complex of deoxycytidylyl-3',5'-guanosine (3',5'-dCpdG) cocrystallized with ribonuclease at 1.9 A resolution.
Acta Crystallogr.,Sect.D, 51:767-771, 1995

PubMed Abstract: The X-ray structure of bovine ribonuclease A cocrystallized with the dinucleotide deoxycytidylyl-3',5'-guanosine has been determined at 1.9 A resolution and refined by restrained least squares to R = 0.218 for 7807 reflections. The structure established that the recently observed retrobound mode of attachment of substrate analogues cytidylyl-2',5'-guanosine and deoxycytidylyl-3',5'-guanosine found in soaked RNase A crystals is also present in the cocrystallized complex. Retrobinding is thus unlikely to be the result of restrictions imposed by the crystalline environment as the ligands soak into the lattice but rather a phenomenon specific to small nucleotides containing guanine.

PubMed: 15299807
DOI: 10.1107/S0907444995001570

Experimental method

X-RAY DIFFRACTION (1.9 Å)