1PNN

PEPTIDE NUCLEIC ACID (PNA) COMPLEXED WITH DNA

Summary for 1PNN

• Entry DOI: 10.2210/pdb1pnn/pdb
• Descriptor: PNA (NH2-P(*C*T*C*T*T*C*T*T*C-HIS-GLY-SER-SER-GLY-HIS-C*T*T*C*T*T*C*T*C)-COOH), DNA (5'-D(GP*AP*AP*GP*AP*AP*GP*AP*G)-3') (3 entities in total)
• Functional Keywords: hairpin pna:dna triplex, triplex watson-crick hoogsteen, peptide nucleic acid-dna complex, peptide nucleic acid/dna
• Total number of polymer chains: 4
• Total formula weight: 16319.94

Authors

Betts, L.,Veal, J.M. (deposition date: 1995-10-13, release date: 1996-03-08, Last modification date: 2023-11-15)

Primary citation

Betts, L.,Josey, J.A.,Veal, J.M.,Jordan, S.R.
A Nucleic Acid Triple Helix Formed by a Peptide Nucleic Acid-DNA Complex
Science, 270:1838-1841, 1995

PubMed Abstract: The crystal structure of a nucleic acid triplex reveals a helix, designated P-form, that differs from previously reported nucleic acid structures. The triplex consists of one polypurine DNA strand complexed to a polypyrimidine hairpin peptide nucleic acid (PNA) and was successfully designed to promote Watson-Crick and Hoogsteen base pairing. The P-form helix is underwound, with a base tilt similar to B-form DNA. The bases are displaced from the helix axis even more than in A-form DNA. Hydrogen bonds between the DNA backbone and the Hoogsteen PNA backbone explain the observation that polypyrimidine PNA sequences form highly stable 2:1 PNA-DNA complexes. This structure expands the number of known stable helical forms that nucleic acids can adopt.

PubMed: 8525381

Experimental method

X-RAY DIFFRACTION (2.5 Å)