1NOR

TWO-DIMENSIONAL 1H-NMR STUDY OF THE SPATIAL STRUCTURE OF NEUROTOXIN II FROM NAJA OXIANA

Summary for 1NOR

• Entry DOI: 10.2210/pdb1nor/pdb
• NMR Information: BMRB: 5690
• Descriptor: NEUROTOXIN II (1 entity in total)
• Functional Keywords: neurotoxin
• Biological source: Naja oxiana (Central Asian cobra)
• Cellular location: Secreted : P01427
• Total number of polymer chains: 1
• Total formula weight: 6895.78

Authors

Golovanov, A.P.,Utkin, Y.N.,Lomize, A.L.,Tsetlin, V.I.,Arseniev, A.S. (deposition date: 1993-04-05, release date: 1993-10-31, Last modification date: 2024-11-20)

Primary citation

Golovanov, A.P.,Lomize, A.L.,Arseniev, A.S.,Utkin, Y.N.,Tsetlin, V.I.
Two-dimensional 1H-NMR study of the spatial structure of neurotoxin II from Naja naja oxiana.
Eur.J.Biochem., 213:1213-1223, 1993

PubMed Abstract: The spatial structure of neurotoxin II from the venom of the central Asian cobra Naja naja oxiana was determined by two-dimensional 1H-NMR techniques and computational analysis. Nearly complete proton resonance assignments for 61 amino acid residues have been made using two-dimensional (2D) homonuclear total correlated spectroscopy, 2D homonuclear double-quantum-filtered correlated spectroscopy and 2D homonuclear NOE spectroscopy (NOESY) experiments. The cross-peak volumes in NOESY spectra spin-spin coupling constants of vicinal protons NH-C alpha H and C alpha H-C beta H and the observation of slow deuterium exchange of amide protons were used to define local structure and a set of constraints for distance geometry program DIANA. The average root-mean-square deviations are 53 pm for backbone heavy atoms and 118 pm for all heavy atoms of 19 final neurotoxin II conformations. The spatial structure is characterized by a short double-stranded (residues 1-5 and 13-17) and a triple-stranded (residues 22-30, 33-41 and 50-54) antiparallel beta-sheets.

PubMed: 8504813
DOI: 10.1111/j.1432-1033.1993.tb17872.x

Experimental method

SOLUTION NMR