1L2Q

Crystal Structure of the Methanosarcina barkeri Monomethylamine Methyltransferase (MtmB)

Summary for 1L2Q

Entry DOI	10.2210/pdb1l2q/pdb
Related	1L2R
Descriptor	monomethylamine methyltransferase, AMMONIUM ION (3 entities in total)
Functional Keywords	tim barrel, transferase
Biological source	Methanosarcina barkeri
Total number of polymer chains	1
Total formula weight	50326.30
Authors	Hao, B.,Gong, W.,Ferguson, T.K.,James, C.M.,Krzycki, J.A.,Chan, M.K. (deposition date: 2002-02-24, release date: 2002-06-05, Last modification date: 2025-03-26)
Primary citation	Hao, B.,Gong, W.,Ferguson, T.K.,James, C.M.,Krzycki, J.A.,Chan, M.K. A new UAG-encoded residue in the structure of a methanogen methyltransferase. Science, 296:1462-1466, 2002 Cited by PubMed Abstract: Genes encoding methanogenic methylamine methyltransferases all contain an in-frame amber (UAG) codon that is read through during translation. We have identified the UAG-encoded residue in a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase (MtmB). This structure reveals a homohexamer comprised of individual subunits with a TIM barrel fold. The electron density for the UAG-encoded residue is distinct from any of the 21 natural amino acids. Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine. PubMed: 12029132 DOI: 10.1126/science.1069556 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.7 Å)

Structure validation