1JE9

NMR SOLUTION STRUCTURE OF NT2

Summary for 1JE9

• Entry DOI: 10.2210/pdb1je9/pdb
• NMR Information: BMRB: 5052,5690
• Descriptor: SHORT NEUROTOXIN II (1 entity in total)
• Functional Keywords: all beta sheet protein, postsynaptic neurotoxin, veonm, toxin
• Biological source: Naja kaouthia (monocled cobra)
• Cellular location: Secreted: P59276
• Total number of polymer chains: 1
• Total formula weight: 6869.75

Authors

Cheng, Y.,Wang, W.,Wang, J. (deposition date: 2001-06-16, release date: 2001-07-04, Last modification date: 2024-10-23)

Primary citation

Cheng, Y.,Meng, Q.,Wang, W.,Wang, J.
Structure-function relationship of three neurotoxins from the venom of Naja kaouthia: a comparison between the NMR-derived structure of NT2 with its homologues, NT1 and NT3
BIOCHIM.BIOPHYS.ACTA, 1594:353-363, 2002

PubMed Abstract: Three homologous short-chain neurotoxins, named NT1, NT2 and NT3, were purified from the venom of Naja kaouthia. NT1 has an identical amino acid sequence to cobrotoxin from Naja naja atra [Biochemistry 32 (1993) 2131]. NT3 shares the same sequence with cobrotoxin b [J. Biochem. (Tokyo) 122 (1997) 1252], whereas NT2 is a novel 61-residue neurotoxin. Tests of their physiological functions indicate that NT1 shows a greater inhibition of muscle contraction induced by electrical stimulation of the nerve than do NT2 and NT3. Homonuclear proton two-dimensional NMR methods were utilized to study the solution tertiary structure of NT2. A homology model-building method was employed to predict the structure of NT3. Comparison of the structures of these three toxins shows that the surface conformation of NT1 facilitates the substituted base residues, Arg28, Arg30, and Arg36, to occupy the favorable spatial location in the central region of loop II, and the cation groups of all three arginines face out of the molecular surface of NT1. This may contribute greatly to the higher binding of NT1 with AchR compared to NT2 and NT3.

PubMed: 11904231
DOI: 10.1016/S0167-4838(01)00326-0

Experimental method

SOLUTION NMR