1J5D
SOLUTION STRUCTURE OF OXIDIZED PARAMAGNETIC CU(II) PLASTOCYANIN FROM SYNECHOCYSTIS PCC6803-MINIMIZED AVERAGE STRUCTURE
Replaces: 1I0YSummary for 1J5D
| Entry DOI | 10.2210/pdb1j5d/pdb |
| Related | 1J5C |
| Descriptor | PLASTOCYANIN, COPPER (II) ION (2 entities in total) |
| Functional Keywords | copper protein beta barrel electron transfer, electron transport |
| Biological source | Synechocystis sp. PCC 6803 |
| Total number of polymer chains | 1 |
| Total formula weight | 10312.98 |
| Authors | Bertini, I.,Ciurli, S.,Dikiy, A.,Fernandez, C.O.,Luchinat, C.,Safarov, N.,Shumilin, S.,Vila, A.J. (deposition date: 2002-04-02, release date: 2002-04-10, Last modification date: 2023-12-27) |
| Primary citation | Bertini, I.,Ciurli, S.,Dikiy, A.,Fernandez, C.O.,Luchinat, C.,Safarov, N.,Shumilin, S.,Vila, A.J. The first solution structure of a paramagnetic copper(II) protein: the case of oxidized plastocyanin from the cyanobacterium Synechocystis PCC6803. J.Am.Chem.Soc., 123:2405-2413, 2001 Cited by PubMed Abstract: The NMR solution structure of oxidized plastocyanin from the cyanobacterium Synechocystis PCC6803 is here reported. The protein contains paramagnetic copper(II), whose electronic relaxation times are quite unfavorable for NMR solution studies. The structure has been solved on the basis of 1041 meaningful NOESY cross-peaks, 18 1D NOEs, 26 T(1) values, 96 dihedral angle constraints, and 18 H-bonds. The detection of broad hyperfine-shifted signals and their full assignment allowed the identification of the copper(II) ligands and the determination of the Cu-S-C-H dihedral angle for the coordinated cysteine. The global root-mean-square deviation from the mean structure for the solution structure family is 0.72 +/- 0.14 and 1.16 +/- 0.17 A for backbone and heavy atoms, respectively. The structure is overall quite satisfactory and represents a breakthrough, in that it includes paramagnetic copper proteins among the metalloproteins for which solution structures can be afforded. The comparison with the available X-ray structure of a triple mutant is also performed. PubMed: 11456890DOI: 10.1021/ja0033685 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
Download full validation report
