1FJE
SOLUTION STRUCTURE OF NUCLEOLIN RBD12 IN COMPLEX WITH SNRE RNA
Summary for 1FJE
| Entry DOI | 10.2210/pdb1fje/pdb |
| Related | 1FJ7 1FJC |
| Descriptor | SNRE RNA, NUCLEOLIN RBD12 (2 entities in total) |
| Functional Keywords | rnp, rbd, rrm, rna binding domain, rna-protein complex, nucleolus, structural protein-rna complex, structural protein/rna |
| Biological source | Mesocricetus auratus (golden hamster) More |
| Total number of polymer chains | 2 |
| Total formula weight | 26461.07 |
| Authors | Allain, F.H.T.,Bouvet, P.,Dieckmann, T.,Feigon, J. (deposition date: 2000-08-08, release date: 2001-01-03, Last modification date: 2024-05-22) |
| Primary citation | Allain, F.H.,Bouvet, P.,Dieckmann, T.,Feigon, J. Molecular basis of sequence-specific recognition of pre-ribosomal RNA by nucleolin. EMBO J., 19:6870-6881, 2000 Cited by PubMed Abstract: The structure of the 28 kDa complex of the first two RNA binding domains (RBDs) of nucleolin (RBD12) with an RNA stem-loop that includes the nucleolin recognition element UCCCGA in the loop was determined by NMR spectroscopy. The structure of nucleolin RBD12 with the nucleolin recognition element (NRE) reveals that the two RBDs bind on opposite sides of the RNA loop, forming a molecular clamp that brings the 5' and 3' ends of the recognition sequence close together and stabilizing the stem-loop. The specific interactions observed in the structure explain the sequence specificity for the NRE sequence. Binding studies of mutant proteins and analysis of conserved residues support the proposed interactions. The mode of interaction of the protein with the RNA and the location of the putative NRE sites suggest that nucleolin may function as an RNA chaperone to prevent improper folding of the nascent pre-rRNA. PubMed: 11118222DOI: 10.1093/emboj/19.24.6870 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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