1ELR

Crystal structure of the TPR2A domain of HOP in complex with the HSP90 peptide MEEVD

Summary for 1ELR

• Entry DOI: 10.2210/pdb1elr/pdb
• Related: 1ELW
• Descriptor: TPR2A-DOMAIN OF HOP, HSP90-PEPTIDE MEEVD, NICKEL (II) ION, ... (4 entities in total)
• Functional Keywords: hop, tpr-domain, peptide-complex, helical repeat, hsp90, protein binding, chaperone
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm : P31948
• Total number of polymer chains: 2
• Total formula weight: 16230.96

Authors

Scheufler, C.,Brinker, A.,Hartl, F.U.,Moarefi, I. (deposition date: 2000-03-14, release date: 2000-04-26, Last modification date: 2024-11-13)

Primary citation

Scheufler, C.,Brinker, A.,Bourenkov, G.,Pegoraro, S.,Moroder, L.,Bartunik, H.,Hartl, F.U.,Moarefi, I.
Structure of TPR domain-peptide complexes: critical elements in the assembly of the Hsp70-Hsp90 multichaperone machine.
Cell(Cambridge,Mass.), 101:199-210, 2000

PubMed Abstract: The adaptor protein Hop mediates the association of the molecular chaperones Hsp70 and Hsp90. The TPR1 domain of Hop specifically recognizes the C-terminal heptapeptide of Hsp70 while the TPR2A domain binds the C-terminal pentapeptide of Hsp90. Both sequences end with the motif EEVD. The crystal structures of the TPR-peptide complexes show the peptides in an extended conformation, spanning a groove in the TPR domains. Peptide binding is mediated by electrostatic interactions with the EEVD motif, with the C-terminal aspartate acting as a two-carboxylate anchor, and by hydrophobic interactions with residues upstream of EEVD. The hydrophobic contacts with the peptide are critical for specificity. These results explain how TPR domains participate in the ordered assembly of Hsp70-Hsp90 multichaperone complexes.

PubMed: 10786835
DOI: 10.1016/S0092-8674(00)80830-2

Experimental method

X-RAY DIFFRACTION (1.9 Å)