1ED7

SOLUTION STRUCTURE OF THE CHITIN-BINDING DOMAIN OF BACILLUS CIRCULANS WL-12 CHITINASE A1

Summary for 1ED7

• Entry DOI: 10.2210/pdb1ed7/pdb
• NMR Information: BMRB: 4588
• Descriptor: CHITINASE A1 (1 entity in total)
• Functional Keywords: twisted beta-sandwich, hydrolase
• Biological source: Bacillus circulans
• Total number of polymer chains: 1
• Total formula weight: 5038.63

Authors

Ikegami, T.,Okada, T.,Hashimoto, M.,Seino, S.,Watanabe, T.,Shirakawa, M. (deposition date: 2000-01-27, release date: 2000-05-24, Last modification date: 2024-05-22)

Primary citation

Ikegami, T.,Okada, T.,Hashimoto, M.,Seino, S.,Watanabe, T.,Shirakawa, M.
Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1.
J.Biol.Chem., 275:13654-13661, 2000

PubMed Abstract: The three-dimensional structure of the chitin-binding domain (ChBD) of chitinase A1 (ChiA1) from a Gram-positive bacterium, Bacillus circulans WL-12, was determined by means of multidimensional heteronuclear NMR methods. ChiA1 is a glycosidase that hydrolyzes chitin and is composed of an N-terminal catalytic domain, two fibronectin type III-like domains, and C-terminal ChBD(ChiA1) (45 residues, Ala(655)-Gln(699)), which binds specifically to insoluble chitin. ChBD(ChiA1) has a compact and globular structure with the topology of a twisted beta-sandwich. This domain contains two antiparallel beta-sheets, one composed of three strands and the other of two strands. The core region formed by the hydrophobic and aromatic residues makes the overall structure rigid and compact. The overall topology of ChBD(ChiA1) is similar to that of the cellulose-binding domain (CBD) of Erwinia chrysanthemi endoglucanase Z (CBD(EGZ)). However, ChBD(ChiA1) lacks the three aromatic residues aligned linearly and exposed to the solvent, which probably interact with cellulose in CBDs. Therefore, the binding mechanism of a group of ChBDs including ChBD(ChiA1) may be different from that proposed for CBDs.

PubMed: 10788483
DOI: 10.1074/jbc.275.18.13654

Experimental method

SOLUTION NMR