1BDJ
COMPLEX STRUCTURE OF HPT DOMAIN AND CHEY
Summary for 1BDJ
| Entry DOI | 10.2210/pdb1bdj/pdb |
| Descriptor | CHEY, AEROBIC RESPIRATION CONTROL SENSOR PROTEIN ARCB, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | two-component system, histidine-containing phosphotransfer (hpt) domain, arcb, response regulator, chey, complex (chemotaxis-transferase), complex (chemotaxis-transferase) complex, complex (chemotaxis/transferase) |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 2 |
| Total formula weight | 28091.14 |
| Authors | Kato, M.,Mizuno, T.,Shimizu, T.,Hakoshima, T. (deposition date: 1998-05-10, release date: 1999-05-11, Last modification date: 2024-05-22) |
| Primary citation | Kato, M.,Shimizu, T.,Mizuno, T.,Hakoshima, T. Structure of the histidine-containing phosphotransfer (HPt) domain of the anaerobic sensor protein ArcB complexed with the chemotaxis response regulator CheY. Acta Crystallogr.,Sect.D, 55:1257-1263, 1999 Cited by PubMed Abstract: The three-dimensional structure of the HPt domain of ArcB complexed with CheY has been determined using the molecular-replacement method. The structure was refined to a crystallographic R factor of 18.3% at 2.68 A resolution. The final model included 1899 protein atoms (117 residues from the HPt domain and 128 residues from CheY), one sulfate ion and 44 solvent molecules. In the crystal, CheY molecules stacked along the a axis of the cell with no interactions between neighbouring rows and the HPt domain bridged the CheY molecules. The phosphodonor residue His715 was fully exposed to the solvent region, even though the HPt domain was in contact with four molecules of CheY. CheY showed significant conformational change. This indicates that the HPt domain has a rigid structure when complexed with CheY. PubMed: 10393292DOI: 10.1107/S0907444999005053 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.68 Å) |
Structure validation
Download full validation report
