191D
CRYSTAL STRUCTURE OF INTERCALATED FOUR-STRANDED D(C3T)
Summary for 191D
| Entry DOI | 10.2210/pdb191d/pdb |
| Descriptor | DNA (5'-D(*CP*CP*CP*T)-3'), SODIUM ION (3 entities in total) |
| Functional Keywords | u-dna, quadruple helix, parallel-stranded tetraplex, base intercalated, mismatched, dna |
| Total number of polymer chains | 4 |
| Total formula weight | 4576.09 |
| Authors | Kang, C.,Berger, I.,Lockshin, C.,Ratliff, R.,Moyzis, R.,Rich, A. (deposition date: 1994-09-29, release date: 1994-11-30, Last modification date: 2024-02-07) |
| Primary citation | Kang, C.H.,Berger, I.,Lockshin, C.,Ratliff, R.,Moyzis, R.,Rich, A. Crystal structure of intercalated four-stranded d(C3T) at 1.4 angstroms resolution. Proc.Natl.Acad.Sci.USA, 91:11636-11640, 1994 Cited by PubMed Abstract: The crystal structure of d(C3T), solved at 1.4 A resolution, reveals that the molecule forms a four-stranded intercalated complex. It consists of two parallel-stranded duplexes, each of which is held together by cytosine-protonated cytosine base pairs. The two duplexes are intercalated with each other and have opposite strand orientation. The molecule has a flat, lath-like appearance, and the covalently bonded cytosines have a slow right-handed twist of 17.1 degrees. However, there is considerable asymmetry. On one of the flat sides, the phosphate groups are rotated away from the center of the molecule. They are held in this orientation by bridging water molecules that bind the NH of cytosine and a phosphate group of an opposite chain. There is also considerable microheterogeneity in the structure. The cytosine hemiprotonation occurs even at pH 7 where stable crystals form. PubMed: 7972115DOI: 10.1073/pnas.91.24.11636 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.4 Å) |
Structure validation
Download full validation report
