191D

CRYSTAL STRUCTURE OF INTERCALATED FOUR-STRANDED D(C3T)

191D の概要

• エントリーDOI: 10.2210/pdb191d/pdb
• 分子名称: DNA (5'-D(*CP*CP*CP*T)-3'), SODIUM ION (3 entities in total)
• 機能のキーワード: u-dna, quadruple helix, parallel-stranded tetraplex, base intercalated, mismatched, dna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 4576.09

構造登録者

Kang, C.,Berger, I.,Lockshin, C.,Ratliff, R.,Moyzis, R.,Rich, A. (登録日: 1994-09-29, 公開日: 1994-11-30, 最終更新日: 2024-02-07)

主引用文献

Kang, C.H.,Berger, I.,Lockshin, C.,Ratliff, R.,Moyzis, R.,Rich, A.
Crystal structure of intercalated four-stranded d(C3T) at 1.4 angstroms resolution.
Proc.Natl.Acad.Sci.USA, 91:11636-11640, 1994

PubMed Abstract: The crystal structure of d(C3T), solved at 1.4 A resolution, reveals that the molecule forms a four-stranded intercalated complex. It consists of two parallel-stranded duplexes, each of which is held together by cytosine-protonated cytosine base pairs. The two duplexes are intercalated with each other and have opposite strand orientation. The molecule has a flat, lath-like appearance, and the covalently bonded cytosines have a slow right-handed twist of 17.1 degrees. However, there is considerable asymmetry. On one of the flat sides, the phosphate groups are rotated away from the center of the molecule. They are held in this orientation by bridging water molecules that bind the NH of cytosine and a phosphate group of an opposite chain. There is also considerable microheterogeneity in the structure. The cytosine hemiprotonation occurs even at pH 7 where stable crystals form.

PubMed: 7972115
DOI: 10.1073/pnas.91.24.11636

実験手法

X-RAY DIFFRACTION (1.4 Å)