183D

X-RAY STRUCTURE OF A DNA DECAMER CONTAINING 7, 8-DIHYDRO-8-OXOGUANINE

183D の概要

• エントリーDOI: 10.2210/pdb183d/pdb
• 分子名称: DNA (5'-D(*CP*CP*AP*(8OG)P*CP*GP*CP*TP*GP*G)-3') (2 entities in total)
• 機能のキーワード: b-dna, double helix, modified, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3061.99

構造登録者

Lipscomb, L.A.,Peek, M.E.,Morningstar, M.L.,Verghis, S.M.,Miller, E.M.,Rich, A.,Essigmann, J.M.,Williams, L.D. (登録日: 1994-08-01, 公開日: 1995-02-27, 最終更新日: 2024-02-07)

主引用文献

Lipscomb, L.A.,Peek, M.E.,Morningstar, M.L.,Verghis, S.M.,Miller, E.M.,Rich, A.,Essigmann, J.M.,Williams, L.D.
X-ray structure of a DNA decamer containing 7,8-dihydro-8-oxoguanine.
Proc.Natl.Acad.Sci.USA, 92:719-723, 1995

PubMed Abstract: We have determined the x-ray structure of a DNA fragment containing 7,8-dihydro-8-oxoguanine (G(O)). The structure of the duplex form of d(CCAGOCGCTGG) has been determined to 1.6-A resolution. The results demonstrate that GO forms Watson-Crick base pairs with the opposite C and that G(O) is in the anti conformation. Structural perturbations induced by C.G(O)anti base pairs are subtle. The structure allows us to identify probable elements by which the DNA repair protein MutM recognizes its substrates. Hydrogen bond donors/acceptors within the major groove are the most likely element. In that groove, the pattern of hydrogen-bond donors/acceptors of C.G(O)anti is unique. Additional structural analysis indicates that conversion of G to G(O) would not significantly influence the glycosidic torsion preference of the nucleoside. There is no steric interaction of the 8-oxygen of G(O) with the phospho-deoxyribose backbone.

PubMed: 7846041
DOI: 10.1073/pnas.92.3.719

実験手法

X-RAY DIFFRACTION (1.6 Å)