144L

ROLE OF BACKBONE FLEXIBILITY IN THE ACCOMMODATION OF VARIANTS THAT REPACK THE CORE OF T4 LYSOZYME

Summary for 144L

• Entry DOI: 10.2210/pdb144l/pdb
• Descriptor: T4 LYSOZYME, CHLORIDE ION, BETA-MERCAPTOETHANOL, ... (4 entities in total)
• Functional Keywords: hydrolase(o-glycosyl)
• Biological source: Enterobacteria phage T4
• Cellular location: Host cytoplasm : P00720
• Total number of polymer chains: 1
• Total formula weight: 18954.69

Authors

Baldwin, E.,Matthews, B.W. (deposition date: 1993-10-15, release date: 1994-01-31, Last modification date: 2024-02-07)

Primary citation

Baldwin, E.P.,Hajiseyedjavadi, O.,Baase, W.A.,Matthews, B.W.
The role of backbone flexibility in the accommodation of variants that repack the core of T4 lysozyme.
Science, 262:1715-1718, 1993

PubMed Abstract: To understand better how the packing of side chains within the core influences protein structure and stability, the crystal structures were determined for eight variants of T4 lysozyme, each of which contains three to five substitutions at adjacent interior sites. Concerted main-chain and side-chain displacements, with movements of helical segments as large as 0.8 angstrom, were observed. In contrast, the angular conformations of the mutated side chains tended to remain unchanged, with torsion angles within 20 degrees of those in the wild-type structure. These observations suggest that not only the rotation of side chains but also movements of the main chain must be considered in the evaluation of which amino acid sequences are compatible with a given protein fold.

PubMed: 8259514

Experimental method

X-RAY DIFFRACTION (2.1 Å)